Summary

The Lee lab is searching for a Research Technician III, who will join our team to help characterize pain-mediating innervation changes in the knee joint that are induced by aging, joint disease, and/or exercise. The Research Technician III will be responsible for performing exercise and behavioral experiments with mouse models of joint disease, as well as tissue clearing and 3D imaging experiments of mouse knee joints. This includes, but is not limited to,  tissue collection, fixation,  decalcification, tissue clearing, immunostaining, and whole-mount 3D imaging using lightsheet fluorescence microscopy.
 

Job Duties

• Conducts tissue clearing and whole-mount 3D imaging experiments.
• Conducts exercise and behavioral experiments.
• Performs tissue clearing on knee joints from various mouse models of joint diseases.
• Optimizes whole-mount in situ histochemistry protocols for cleared mouse knee joints.
• Optimizes and performs post-aquisition image analyses.
• Plans and conducts exercise experiments (treadmill) and behavioral experiments (Von Frey).
• Supports tissue collections (includes perfusion).

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• One year of relevant experience.

Preferred Qualifications

• Master's degree in basic science or a related field.
• Background in tissue clearing and 3D imaging.
• Experience working with mouse models.