H. Lee Moffitt Cancer Center

Tampa, FL

Job posting number: #7131007 (Ref:hlj_43852)

Posted: March 15, 2023

Application Deadline: Open Until Filled

Job Description


The laboratory of Dr. Jiandong Chen at Moffitt Cancer Center Department of Molecular Oncology conducts basic research to understand the p53 tumor suppressor pathway and development of novel therapeutic strategies to target p53 for cancer therapy. The lab routinely performs mechanistic studies of protein expression, function, and reguation in response to novel drugs and stress treatments using cell culture and mouse models. The lab has extensive collaborations with chemists and structural biologists working in drug discovery, providing a rich environment for training and career development.

Position Highlights:

  • Analysis of novel compounds from chemistry collaborators.
  • Use mouse models to investigate efficacy of novel cancer drugs.
  • Cell culture, CRISPR/Cas9 gene editing, creation of stable and inducible cell lines.
  • Provides training in multiple techniques required by the projects.

The Ideal Candidate:

  • Experience with cell culture and bacteria culture.
  • Experience with Western blot, PCR, RT-PCR, FACS.
  • Experience with recombinant DNA techniques.
  • Experience with mouse xenograft models or genetically modified mice.
  • Proficient in calculating drug molar concentrations.


  • Cell culture and drug treatment.
  • Analysis of samples by Western blot, RT-PCR.
  • Molecular cloning, plasmid purification.
  • Recombinant protein expression in bacteria and purification.
  • Perform transfection, reporter assays.
  • Maintain mouse strains by PCR genotyping.
  • Generation and treatment of tumor xenografts.
  • Preparation of buffers and solutions.

Credentials and Qualifications:

  • Bachelor’s degree in biology or related field is required.
  • Hands on research experience required.

Mission To create a Moffitt culture of diversity and inclusion as we strive to contribute to the prevention and cure of cancer. Vision Moffitt Cancer Center is recognized as the model wherein the diversity of our employees and communities is valued and supported as essential components to contributing to the prevention and cure of cancer. The cancer center is an equal opportunity employer. It is the policy of the cancer center to prohibit unlawful discrimination and harassment of any type and to afford equal employment opportunities to workforce members and applicants, regardless of race, color, religion, sex, sexual orientation, gender identity or expression, national origin, age, marital status, disability, genetic information, veteran’s status or any other characteristic protected by federal, state or local law.

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More Info

Job posting number:#7131007 (Ref:hlj_43852)
Application Deadline:Open Until Filled
Employer Location:H. Lee Moffitt Cancer Center
United States
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