Summary

The goal of this project is to model a human disease in fruit flies. The Research Technician II will work with two senior postdoctoral fellows and perform sophisticated cloning experiments to insert human cDNAs in vectors, create variants, deletions, and produce the corresponding transgenic flies using genetic crosses. Will then determine where the genes are expressed by immunostaining and perform the confocal microscopy to assess where the gene is expressed and where the proteins are localized subcellularly. Using genetic crosses other mutants will be intercrossed with these transgenic stocks to suppress or enhance the observed phenotypes. Finally, the data that are observed in flies will be extrapolated in human cultured cells to assess if the models generated in flies are evolutionarily conserved.

Job Duties

• Performs molecular biology experiments, transgenesis experiments with flies, extensive genetic crosses with flies, immunostaining of fly tissues, confocal microscopy, maintains fly stocks.
• Prepares and conducts laboratory research experiments.
• Collects, compiles and analyzes data
• Documents results of experiments.

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• No experience required.

Preferred Qualifications

• Bachelor or Master in biology (Molecular Biology, Genetics, Neuroscience, Cell Biology)