Summary

The position is for an experienced technician to provide research assistance for studies on rotavirus (RV), caliciviruses, and various other BSL-2 viruses in the Department of Molecular Virology and Microbiology. This job is suitable for someone who is highly organized and can learn to follow protocols such that they can work with minimal supervision. Work for this Technician position will encompass both sterile maintenance and culturing of multiple cell lines, virus and viral vector stock production, transfections & transduction to generate cell lines, mouse husbandry, as well as general molecular biology techniques (e.g., western blot, PCR, immunofluorescence). The candidate should have well-developed skills for lab organization, maintaining reagent databases, and reagent ordering. This position will offer a diverse and supportive lab environment and opportunity to contribute to basic virology research in a growing research program.  

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Job Duties

• Maintains cell and human intestinal organoids, grows virus stocks, packages lentiviruses, and generates stable cell lines.
• Orders lab supplies and reagents.
• Organizes and maintains logs for cell lines, virus strains, reagents and freezers.
• Mouse husbandry and assists researchers with animal studies.
• Runs various molecular biology experiments, including western blot, PCR for animal genotyping, RT-qPCR, immunofluorescence staining & microscopy.
• Maintains experimental notebooks and records.

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• No experience required.

Preferred Qualifications

• Masters degree and/or with prior research experience.
• Experience in virology or microbiology is preferred but any experience in molecular biology will be considered.
• Experience with microscopy.
• Strong computer skills with Word, PowerPoint, Excel.
  • Additional computer skills with Adobe Illustrator and Photoshop.