Research Technologist

Johns Hopkins University

Baltimore, MD

Job posting number: #7083983

Posted: August 26, 2021

Application Deadline: Open Until Filled

Job Description

The Department of Genetic Medicine seeks a Research Technologist who will be responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.



Specific Duties & Responsibilities:

Barcode and enter sample associated information into the database.
Transfer DNA samples into 96-well plate format.
Receive and prepare deepwell DNA stock plates and create working DNA plates.
Determine concentration and volume of DNA samples using various instruments.
Dilute samples to working concentrations.
Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
Operate and troubleshoot robotic liquid handlers.
Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina HiSeq workflows.
Perform quality control checks of data using various software, including, but not limited to, GenomeStudio, Spotfire and other in-house QC metrics reports.
Maintain timely and accurate laboratory records (electronic and written).
Collect, organize, interpret and submit experimental data to database and supervisors.
Perform general lab maintenance duties.
Perform validation tests of lab reagents.
Participate in committees that function to improve processes.
Communicate effectively to supervisor and team members.
Perform miscellaneous related duties as assigned.


Minimum Qualifications (Mandatory):

Bachelor’s degree in biology, chemistry or related field.


Preferred Qualifications:

6 months of post-undergraduate experience in a comparable lab or significant research project, not related to normal course requirements.


Special Knowledge, Skills & Abilities:

Basic understanding of molecular biology and genetics.
Ability to safely operate scientific laboratory equipment.
Able to work flexible hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.
Superior organizational skills for time and data management.
Ability to function well in a team production setting.


Machines/Equipment Used:

Centrifuges
Illumina BeadLab system (Tecans, iScans, HiScans, TeFlow)
Illumina BeadXpress
Covaris
BioAnalyzer
Nanodrop instruments
Illumina HiSeq
SpriWorks
Thermal cyclers
Robotic and manual liquid handlers
Common laboratory equipment
Macintosh
PC computers
UNIX servers
Oracle database


Physical Requirements:

Sitting in a normal seated position for extended periods of time.
Reaching by extending hand(s) or arm(s) in any direction.
Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard.
Communication skills using the spoken word.
Ability to see and hear within normal parameters.
Ability to move about workspace.
Lift, carry, move supplies, computers, etc.


Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience)
Employee group: Full Time
Schedule: Mon-Fri hours between 6AM & 6PM
Exempt Status: Non-Exempt
Location: School of Medicine Campus
Department name: ​​​​​​​SOM Genetics Ctr for Inhrtd Dis Res CIDR
Personnel area: School of Medicine



The successful candidate(s) for this position will be subject to a pre-employment background check.



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